Comparison of the Behavior of 3D-Printed Endothelial Cells in Different Bioinks
نویسندگان
چکیده
Biomaterials with characteristics similar to extracellular matrix and suitable bioprinting properties are essential for vascular tissue engineering. In search biomaterials, this study investigated the three hydrogels alginate/hyaluronic acid/gelatin (Alg/HA/Gel), pre-crosslinked alginate di-aldehyde gelatin (ADA-GEL), methacryloyl (GelMA) respect their mechanical survival, migration, proliferation of human umbilical vein endothelial cells (HUVECs). addition, behavior HUVECs was compared in Matrigel. For purpose, were mixed inks both as single cell spheroids printed using extrusion-based bioprinting. Good printability shape fidelity determined all inks. The rheological measurements demonstrated gelling consistency shear-thinning behavior. Different Young’s moduli determined. However, measured values where within range defined literature, leading migration sprouting, well reconciling adhesion. Cell survival ADA-GEL GelMA 14 days. Alg/HA/Gel bioink, death occurred 7 days cells. Sprouting HUVEC observed GelMA. Similar seen contrast, ink died over time studied. It has been shown that does not provide a good environment long-term HUVECs. conclusion, promising
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ژورنال
عنوان ژورنال: Bioengineering
سال: 2023
ISSN: ['2306-5354']
DOI: https://doi.org/10.3390/bioengineering10070751